大鼠急性心肌缺血再灌注Bcl-2蛋白的表达及其法医病理学意义

目的为心肌缺血/再灌注损伤所致心性猝死的法医学诊断提供形态学依据。方法运用免疫组织化学方法,研究Bcl-2蛋白在心肌组织中不同区域的表达。结果对照组正常心肌组织无Bcl-2蛋白表达。Bcl-2蛋白再灌注0.5h后即可在缺血区域表达,且表达主要在心肌内层。再灌注1h后可观察到Bcl-2蛋白不仅表达于心肌内层且已经扩展至心肌的全层。再灌注2h发现心肌外层表达明显强于心肌内层,随着再灌注时间的延长可以发现Bcl-2蛋白的表达逐渐减弱。结论在急性心肌缺血再灌注中,Bcl-2基因对心肌细胞凋亡的发生有着重要的作用,且可以作为心肌缺血再灌注的早期诊断提供一项辅助诊断依据。

The expression of Bcl-2 protein on acute myocardial ischemia/reperfusion and the significance in forensic pathology

Objective To search the objective morphologic evidence for the forensic pathological diagnosis on the acute myocardial ischemia/reperfusion.Methods The expression of Bcl-2protein in the early stage of acute myocardial ischemia/reperfusion(I/R)in rats were studyed with immunohistochemistry.Results There was no expression of Bcl-2protein in control group.The expression of Bcl-2protein began to increase in myocytes0.5h after I/R in the ischemic areas and the expression of Bcl-2protein in inner layer was stronger than in outer layer.The expression of Bcl-2protein appears firstly in the inner layer of my - ocardium and extended gradually toward the outer layer1h after I/R.It was found that the expression of Bcl-2protein in outer layer was stronger than in inner layer,then the expression of Bcl-2protein be - came weaken gradually.Conclusion Bcl-2gene may be an important regulative gene in the process of apoptosis and the changes observed in this paper might be useful for the forensic pathological diagnosis on the early myocardial ischemia.