A method for the identification and typing of the subtypes of the Gc 1 allele from dried bloodstains |
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| Authors: | M Baxter I White |
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| Affiliation: | 1. Strathclyde Police Headquarters Forensic Science Laboratory, 173 Pitt Street, Glasgow, United Kingdom G2 4JS;2. Department of Human Genetics, University of Newcastle, 19 Claremont Place, Newcastle upon Tyne, United Kingdom NG1 7RU;1. Department of Transfusion Medicine Medanta-The Medicity, Sector-38, Gurgaon, India;2. Department of Clinical Microbiology Institute of Liver and Biliary Sciences, New Delhi, India;3. Department of Microbiology College of Life Sciences, Jiwaji University Gwalior, India;4. Medanta Institute of Education and Research Medanta-The Medicity, Sector-38, Gurgaon, India;5. Independent Laboratory Consultant, Chennai, Tamil Nadu, India;6. Laboratory Medicine, Thane West, Mumbai 400606, India;1. Sheffield Teaching Hospitals NHS Foundation Trust, Glossop Road, Sheffield, S10 2JF, United Kingdom;2. University of Sheffield, Beech Hill Road, Sheffield, S10 2RX, United Kingdom |
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| Abstract: | Subtyping of the 1F and 1S variants of Gc in bloodstains was achieved by isoelectric focusing in ultra-thin polyacrylamide gels using MOPS (morpholinopropanesulfonic acid) spacer in the gel. A blind trial showed no examples of mis-typing of 98 samples. Ninety-three per cent of 1-week-old stains could be grouped, falling to 53% in 12-week-old stains. Citrate was found to have a deleterious effect on stain typing. The gene frequencies calculated from 1675 West of Scotland samples were: 1S, 0·555; 1F, 0·153; 2, 0·292. |
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