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应用DNA IQ^TM SYSTEM检验混合斑的精子DNA
引用本文:夏雨. 应用DNA IQ^TM SYSTEM检验混合斑的精子DNA[J]. 贵州警官职业学院学报, 2007, 19(5): 70-72
作者姓名:夏雨
作者单位:贵州警官职业学院,贵州,贵阳,550005
摘    要:在两步消化法的基础上,采用DNA IQ^TM system提取精子DNA.通过磁性颗粒饱和性吸附.纯化模板DNA.稳定模板浓度,得到清晰完整的DNA分型.结合Biomek3000自动工作站.可有效减少人为因素的不确定性。提高检出率.

关 键 词:DNA  IQ^TM  System  法医物证  混合斑  DNA提取
文章编号:1671-5195(2007)05-0070-03
修稿时间:2007-06-30

On Examining the Sperm DNA of Mixed Stain by Using DNA IQTM System
XIA Yu. On Examining the Sperm DNA of Mixed Stain by Using DNA IQTM System[J]. Journal of Guizhou Police Officer Vocational College, 2007, 19(5): 70-72
Authors:XIA Yu
Affiliation:XIA Yu(Guizhou Police Officer Vocational College,Guiyang 550005,China)
Abstract:Extraction method of the assay was based on conventional two-step method for the extraction of sperm DNA of mixed stain,DNA IQTM system was used for extracting sperm DNA,template DNA adsorbed and purified,stable concentration of DNA can be made by magnetic beads and obtained a clear and integrated DNA genotyping.With DNA automation workstation,the automated magnetic beads-based extraction can reduced the uncertainty of operation and increase successful rate.
Keywords:DNA IQTM system  forensic biological evidence  mixed stain  extraction of DNA
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