| 鸡白痢沙门菌等位基因特异性PCR检测方法的建立 |
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| 引用本文： | 耿士忠,潘志明,蒋春红,张辉,李求春,焦新安. 鸡白痢沙门菌等位基因特异性PCR检测方法的建立[J]. 中国兽医科学, 2007, 37(2): 113-116 |
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| 作者姓名： | 耿士忠 潘志明 蒋春红 张辉 李求春 焦新安 |
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| 作者单位： | 扬州大学,江苏省人兽共患病学重点实验室,江苏,扬州,225009 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金;教育部FANEDD专项资金项目 |
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| 摘 要： | 根据鸡白痢沙门菌与鸡伤寒沙门菌的rfbS基因在第237和第598位碱基的不同,设计和合成了等位基因特异性PCR引物,建立了快速检测鸡白痢沙门菌的PCR方法,并应用该方法对鸡白痢沙门菌临床分离样品进行了PCR鉴定。结果显示,该PCR方法能够特异性地鉴定鸡白痢沙门菌,检测灵敏度达100 pgDNA。对35个经常规方法鉴定的鸡白痢沙门菌分离株应用等位基因特异性PCR方法进行鉴定,鉴定出33株鸡白痢沙门菌,符合率为94.3%。表明,建立的等位基因特异性PCR方法能够准确而快速地鉴定鸡白痢沙门菌。
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| 关 键 词： | 鸡白痢沙门菌 等位基因特异性PCR rfbS基因 |
| 文章编号： | 1673-4696(2007)02-0113-04 |
| 修稿时间： | 2006-09-21 |
Development of allele-specific PCR assay for detecting Salmonella pullorum |
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| GENG Shi-zhong,PAN Zhi-ming,JIANG Chun-hong,ZHANG Hui,LI Qiu-chun,JIAO Xin-an. Development of allele-specific PCR assay for detecting Salmonella pullorum[J]. Chinese Veterinary Science, 2007, 37(2): 113-116 |
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| Authors: | GENG Shi-zhong PAN Zhi-ming JIANG Chun-hong ZHANG Hui LI Qiu-chun JIAO Xin-an |
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| Abstract: | Based on the nuclei polymorphism of rfbS gene sequence of Salmonella pullorum at site 237 and 598 compared with S.gallinarum,a pair of allele-specific primers were designed and synthesized, and an allele-specific PCR method for detecting S.pullorum was developed.The PCR detection result of 35 S.pullorum clinical isolates was 94.3% consistent with result of the serological and biochemical method and the sensitivity of the PCR assay was 100 pg.The results showed that the developed allele-specific PCR is a very rapid,sensitive and specific molecular tool for the detection and identification of S.pullorum isolates. |
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| Keywords: | Salmonella pullorum allele-specific PCR rfbS gene |
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