DNA指纹图在法医学鉴定中应用的研究(Ⅰ)

应用 MYO DNA 探针对中国人进行了 DNA 遗传指纹图检验。从两个家系16人及100个无关个体中取肘静脉血提取 DNA,用限制性内切酶 HinfⅠ或 HaeⅢ水解,1%琼脂糖凝胶电泳分离,经 Southern印迹转移,MYO DNA 探针杂交,获得了清晰可辨的 DNA 指纹图谱。结果每个个体在3.0Kb 以上均能检出10条以上杂交区带,个体间的相关概率<4×10~(-9),由杂交区带构成的图谱是个体特异的,杂交区带遵循孟德尔的显性遗传方式由亲代向子代遗传;具有 DNA Fingerprints 的特点。对两起亲子鉴定的案例进行指纹图检验,孩子所存在的杂交区带,除来自母亲外,其余可在嫌疑父亲带中找到,肯定了孩子与嫌疑人的父子关系。MYO DNA 探针在亲子鉴定与个人识别的法医学鉴定中有着重要的实用价值。

THE PRACTICAL USE OF THE DNA FINGERPRINT IN FORENSIC MEDICINE

DNA fingerprints analysis of Chinese population were conducted by Myo mini-satellite DNA probes with elbow venous blood samples collected from 16 personsof two family and 100 unrelated individuals,Distinct DNA fingerprints were ob-tained when the DNA extracted from blood samples were digested with the Hinf Ⅰor Hae Ⅱ,and Southern blot hybridized with Myo minisatellite probe.Theresulting showed that more than ten hybridized bands could be detected of eachindividual.The chance of band sharing among-the individuals is<4×10~(-9),so thefingerprint patterns obtained Were individual specific.Hybridized bands wereinherited according to the Mendelian dominant fashion.In two paternity cases,some of the hybridized bands could be detected in the maternal side,the rest ofthem could be found in the suspected father's,thus confirmed the suspected fatheras biological father.MYO DNA probes have a great practical value in the paternitytest as well as in the personnal identification.