| 死后组织DNA变化与死亡时间关系的研究 |
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| 引用本文： | 王慧君,阮海根. 死后组织DNA变化与死亡时间关系的研究[J]. 刑事技术, 2000, 0(1): 9-11 |
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| 作者姓名： | 王慧君 阮海根 |
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| 作者单位： | 同济医科大学 武汉430030(王慧君，王先松，饶广勋，黄光照)，武汉市公安局刑科所 430019(阮海根，徐宏平)，武汉市公安局刑科所 430019(胡家伟) |
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| 摘 要： | 为调查死后组织DNA含量变化与死亡时间的关系,本文采用流式细胞仪对死后0～96小时(0～96hourpostmortem,hpm)大鼠的骨骼肌、脾、肾细胞DNA含量进行分析,结果表明,各组织显示了随死后间隔时间延长,含碎片DNA细胞增多,含完整DNA细胞减少的趋势;DNA降解系列组方图分析结果表明,脾组织降解速度明显快于肾和骨骼肌组织;骨骼肌在制成单细胞悬液过程中的人为损伤,可造成DNA提前降解.
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| 关 键 词： | 流式细胞仪 DNA含量 死亡时间 |
A STUDY ON THE RELATIONSHIP BETWEEN THE CHANGES OF DNA CONTENT OF THE SKELETAL MUSCLE, SPLEEN AND KIDNEY TISSUS AND THE TIME OF DEATH |
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| Wang Huijun,Wang Xiansong,Rao Guangxun,et al.. A STUDY ON THE RELATIONSHIP BETWEEN THE CHANGES OF DNA CONTENT OF THE SKELETAL MUSCLE, SPLEEN AND KIDNEY TISSUS AND THE TIME OF DEATH[J]. Forensic Science and Technology, 2000, 0(1): 9-11 |
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| Authors: | Wang Huijun Wang Xiansong Rao Guangxun et al. |
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| Affiliation: | Wang Huijun,Wang Xiansong,Rao Guangxun,et al. Department of ultrastructural pathology,Tongji medical university,wuhan 430030 |
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| Abstract: | In oder to investigete the relationship between the changes of DNA content and time of death (post mortem), the DNA content of skeletal muscle, spleen and kidney were analysed with flow cytometry (0-96 hours post mortem) . The results showed that with prolonging of the post mortem interval the DNA fragments containing cells were increased and the cells with intact DNA were decreased. The analysis of the DNA degradation figures revealed that the degradation of the spleen tissue was markedly rapider than that of the kidney and the skeletal muscle. The artificial damage of the skeletal muscle during the preparation of single cell suspension could cause the earlier degradation of the DNA. |
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| Keywords: | flow cytometry DNA content time of death |
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