采血管中添加剂对血样中乙醇含量的影响

目的：探讨不同种类采血管对血样中乙醇含量检测结果的影响。方法分别用7种一次性真空采血管无抗凝剂管、促凝剂管、分离胶-促凝剂管、枸橼酸钠（1∶4）管、枸橼酸钠（1∶9）管、柠檬酸钠（9∶1）管、EDTA-K2管]采集10名志愿者饮酒后2 h血液，用顶空气相色谱法检测血样中乙醇含量。结果相同血样用不同的采血管，其乙醇含量检测结果不同，依次为分离胶-促凝剂管〉促凝剂管〉无抗凝剂管〉EDTA-K2管〉枸橼酸钠（1∶9）管〉枸橼酸钠（1∶4）管，柠檬酸钠（9∶1）管与枸橼酸钠（1∶9）管检测结果基本一致。结论采集涉嫌酒后驾驶的驾驶员血样，应选用一次性真空采血管，首选EDTA-K2管。

Effects of Additives in Blood Collection Tubes on Testing the Alcohol Concentra-tion in Blood Samples

Objective To discuss blood collection tubes with different additives and their effects on the testing results of alcohol concentration in blood sam ples. Methods Blood sam ples from 10 volunteers were collected 2 hours after drinking with seven different types of disposable vacuum blood collection tubes, including ordinary tube without anticoagulant, coagulant tube, separating gel-coagulant tube, sodi-um citrate （1∶4） tube, sodium citrate （1∶9） tube, sodium citrate （9∶1） tube and EDTA-K2 tube. The al-cohol concentrations in these blood sam ples were analyzed by headspace gas chrom atography. Results The concentration testing results of the sam e blood sam ples in different types of tubes were different from one to another. The sequence was as follows：separating gel-coagulant tube〉coagulant tube〉ordi-nary tube without anticoagulant〉EDTA-K2 tube〉sodium citrate （1∶9） tube〉sodium citrate （1∶4） tube, whereas the results of the sam e blood sam ple in sodium citrate （1∶9） tube and sodium citrate （9∶1） tube showed no obvious difference. Conclusion It is better to collect a suspicious drunk driver’s blood sam-ple using a disposable vacuum blood collection tube, with the EDTA-K2 tube being preferred.