血液中乌头碱、次乌头碱、新乌头碱的LC/MS/MS分析 |
| |
引用本文: | 张润生,余琛,刘罡一,张慧. 血液中乌头碱、次乌头碱、新乌头碱的LC/MS/MS分析[J]. 中国法医学杂志, 2004, 19(5): 265-267 |
| |
作者姓名: | 张润生 余琛 刘罡一 张慧 |
| |
作者单位: | 1. 上海市刑事科学技术研究所,上海,200083 2. 上海市徐汇区中心医院中心实验室,上海,200031 |
| |
摘 要: | 目的 建立血液中乌头碱、次乌头碱、新乌头碱的LC/MS/MS分析方法。方法 用 1%三氯乙酸 乙腈萃取血液样品 ,采用电喷雾离子源 ,正离子MRM扫描。结果 本方法线性相关系数r≥ 0 992 2。最低检测浓度(LOQ ,S/N =5 )分别为乌头碱 2 0ng/ml ,次乌头碱 0 5ng/ml ,新乌头碱 0 5ng/ml。空白血添加回收率 91 2 5 %~10 3 1%,变异系数 (CV ,n =6) <10 93 %。结论 LC/MS/MS法灵敏可靠 ,样品处理快速简便 ,适用于血液中乌头碱、次乌头碱、新乌头碱的检测。
|
关 键 词: | 法医毒物分析 血液 乌头碱 次乌头碱 新乌头碱 LC/MS/MS |
文章编号: | 1001-5728(2004)05-0265-03 |
修稿时间: | 2003-12-01 |
Detection of Aconitine and Mesaconitine and Hypaconitine in blood by LC/MS/MS |
| |
Abstract: | Objective To establish a method used for determination of Aconitine and Hypaconitine and Mesaconitine in blood by LC/MS/MS.Methods Extraction of blood sample was conducted by use of 1% thirdchloroactic acid and acetonitrile liquid,and the electrospray fog ionic source,and positive ion MRM scan were employed.Result The linearity correlation coefficients(r)was≥0.992 2.The limit of quantification (LOQ S/N=5) was 2.0 ng/ml for Aconitine,0.5 ng/ml for Hypaconitine,and 0.5 ng/ml for Mesaconitine.The recovery of appended contrast in blank blood sample ranged from 91.25% to 103.1% with coefficient of variation (CV,n=6) less than 10.93%.Conclusion The method of LC/MS/MS is sensitive and reliable,and the sample handling is quick and simple,which was suitable for determination of the Aconitine and Hypaconitine and Mesaconitine in blood sample. |
| |
Keywords: | Forensic toxicological analysis Blood Aconitine Hypaconitine Mesaconitine LC/MS/MS |
本文献已被 CNKI 万方数据 等数据库收录! |
|