An improved method for semen alpha-L-fucosidase typing--distribution in the Wuhan population of China |
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| Authors: | Q G Yang Q J Huang R Z Yang |
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| Affiliation: | Department of Forensic Serology, Faculty of Forensic Medicine, TongJi Medical University, Wuhan, The People's Republic of China. |
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| Abstract: | An improved method for the separation of the genetic variants of alpha-L-fucosidase in human semen is described. The method involves, isoelectric focusing in ultrathin-layer PAG containing a mixture of ampholines, pH 5-7 and pH 3.5-9.5, and separator HEPES. The Fu pattern obtained is simple, easy to interpret, and reproducible. The occurrence of fucosidase phenotypes in 189 semen samples from the population of Wuhan was investigated, and the frequencies of the Fu alleles were calculated. |
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