| 猪和兔附红细胞体的体外培养 |
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| 引用本文： | 房春林,杨光友.猪和兔附红细胞体的体外培养[J].中国兽医科学,2005,35(3):190-193. |
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| 作者姓名： | 房春林 杨光友 |
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| 作者单位： | 四川农业大学,动物科技学院,动物疫病与人类健康四川省重点实验室,四川,雅安,625014 |
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| 基金项目： | 四川省科技厅应用基础研究项目 (04JY029 006 2)，四川省重点建设学科项目资助(SZD0418) |
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| 摘 要： | 用RPMI1640和小牛血清按不同比例混合作为体外培养猪和兔附红细胞体的基础培养基,同时以去掉红细胞上自然感染的附红细胞体后的红细胞泥作为培养附红细胞体的寄生载体,进行猪和兔附红细胞体的体外培养。培养结果表明,以 RPMI1640 与小牛血清按 6∶4 比例混合,同时选用兔红细胞泥作为载体的培养效果最佳,接种感染了附红细胞体的猪、兔阳性红细胞后 24 h生长达到高峰,感染率分别为40.2%和31.0%,而采用猪红细胞泥作为培养载体时红细胞感染率无明显升高。
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| 关 键 词： | 猪附红细胞体 兔附红细胞体 体外培养 感染率 |
| 文章编号： | 1000-6419(2005)03-0190-04 |
| 修稿时间： | 2004年11月18 |
In vitro culture of Eperythrozoon suis and E.lepus |
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| FANG Chun-lin,YANG Guang-you.In vitro culture of Eperythrozoon suis and E.lepus[J].Veterinary Science in China,2005,35(3):190-193. |
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| Authors: | FANG Chun-lin YANG Guang-you |
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| Abstract: | In vitro culture of Eperythrozoon suis and E.lepus was carried out by using different ratios of RPMI1640 to bovine calf serum and RBC after removing the naturally infected Eperythrozoon. At 24th h post-inoculation , the Eperythrozoon all grew well in the media with 60% RPMI1640, 40% bovine calf serum and rabbit RBC removed Eperythrozoon, the infection rates of RBC were up to 40.2% and 31.0% respectively. In contrast, in the media with 60% RPMI 1640, 40% bovine calf serum and swine RBC removed Eperythrozoon, the E.suis and E.lepus could not grow well. The result showed that the rabbit RBC was suitable for the inoculation of E.suis and E.lepus. |
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| Keywords: | Eperythrozoon suis E lepus in vitro maintenance infection rate |
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