猪生殖与呼吸综合征血清抗体重组N蛋白间接ELISA检测方法的建立

以纯化的猪生殖与呼吸综合征病毒 (PRRSV)重组N蛋白作为包被抗原 ,通过对各项试验条件的优化 ,建立起检测PRRS血清抗体的重组N蛋白间接ELISA检测方法。用此方法检测了2 0 0份血清样品 ,并与IDEXX公司ELISA试剂盒的检测结果相比较 ,敏感性和特异性分别为 93%和95 % ,总符合率达 91%。 2种检测方法的结果之间差异不显著 (P >0 .0 5 )。试验表明 ,建立的间接ELISA检测方法具有敏感性高、特异性强、重复性好的优点 ,可用于PRRS血清抗体的检测。

Development of indirect ELISA for detection of antibody to porcine reproductive and respiratory syndrome virus in swine sera using recombinant E.coli-expressed N protein as antigen

Using purified recombinant nucleocapsid protein (N protein) as coated antigen, the reaction conditions of an indirect ELISA were optimized, and then the indirect ELISA was developed for detection of antibodies to porcine reproductive and respiratory syndrome virus (PRRSV ). 200 serum samples were detected by the method and IDEXX ELISA kit respectively. Compared with the IDEXX ELISA kit,the sensitivity and specificity of the ELISAwere 93% and 95% respectively, and the total coincident rate (between) the two methodsreached at 91%. No significationdifference was found between the two methods(P>0.05).The results showed that the developed indirect ELISA had the advantages such as high sensibility, strong specificity and good repeatability and the method can be used to detect the porcine serum (antibodies) to PRRSV.