| 二醋吗啡对大鼠心肌细胞游离Ca^2＋浓度的作用 |
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| 作者姓名： | Liu XS Wu JD Hao ZR Liu SP Li ZH Li HX Chen YC |
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| 作者单位： | 1. 中山大学中山医学院法医系,广东,广州,510080
2. 中山市公安局石歧分局,广东,中山,528403 |
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| 摘 要： | 目的观察二醋吗啡对心肌细胞内游离Ca2 浓度的作用。方法取自1～3d的SD大鼠培养的心肌细胞,用荧光探针Fluo-3/AM负载心肌细胞,不同浓度及不同剂量的二醋吗啡作用心肌细胞,激光共聚焦显微镜下检测Ca2 的变化。结果不同剂量及浓度的二醋吗啡对心肌细胞内游离Ca2 浓度有不同作用,一定浓度的二醋吗啡使心肌细胞内游离Ca2 浓度呈剂量依赖性的升高、短时间内心肌细胞Ca2 ]i荧光强度增强,并产生Ca2 ]i峰。结论探索出二醋吗啡导致心肌细胞内Ca2 变化的有效浓度,为进一步深入研究打下了基础。
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| 关 键 词： | 二醋吗啡 心肌细胞 游离Ca2 |
| 文章编号： | 1004-5619(2007)06-0424-04 |
| 修稿时间： | 2007年1月29日 |
The effects of heroin on intracellular free Ca2+ of rat myocardium |
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| Liu XS,Wu JD,Hao ZR,Liu SP,Li ZH,Li HX,Chen YC.The effects of heroin on intracellular free Ca2+ of rat myocardium[J].Journal of Forensic Medicine,2007,23(6):424-427. |
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| Authors: | Liu Xiao-Shan Wu Jing-Dong Hao Zi-Rui Liu Shui-Ping Li Zhao-Hui Li Hai-Xia Chen Yu-Chuan |
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| Institution: | Department of Forensic Medicine, Medical College of Sun Yat-sen University, Guangzhou, China. lxsa200@yahoo.com.cn |
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| Abstract: | Objective To observe the effects of heroin on intracellular free Ca2 in rat myocardium. Methods The effects of heroin on intracellular free Ca2 were observed in cultured neonatal rat myocardium by measuring intracellular free Ca2 concentration using calcium fluorescent probe Flou-3/AM and laser scanning confocal microscope. Results Different doses and concentrations of heroin appeared to have different effects on intracellular free Ca2 concentrations, with a dosage dependent short linear increase in the fluorescence intensity (i.e., Ca2 concentration) leading to Ca2 ]i peak. Conclusion Heroin could affect concentrations of Ca2 ]i in myocardium and its dosage related effect needs further investigation. |
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| Keywords: | heroin myocardial cells free Ca2 |
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