毒鼠强中毒的凋亡细胞研究

目的通过动物实验,研究观察在不同剂量下毒鼠强中毒后不同时间各器官的病理学变化,以期为法医学鉴定提供指标。方法建立小鼠毒鼠强中毒的实验模型,通过常规H.E染色及凋亡细胞检测技术对小鼠毒鼠强急、慢性中毒后脑、心、肝、肾等器官的病理学改变进行系统研究,并对研究结果进行计算机病理学图像分析;对中毒实验组的小鼠上述器官检材进行毒物分析。结果慢性中毒组小鼠在中毒后的不同时间段内,脑、心、肝、肾等器官凋亡细胞数均高于正常对照组及急性中毒致死组,且同一器官的凋亡细胞数在不同中毒时间段内有所差异,差别均有显著性;不同器官的凋亡细胞数的峰值在不同中毒时间段内亦有所不同。急性中毒组小鼠各器官的毒物分析结果为阳性,慢性中毒组均为阴性。结论慢性毒鼠强中毒,在临床症状不明显及法医毒物分析难以检测的情况下,应用凋亡细胞检测技术可成功检测出机体的主要器官的病理学改变,表明小剂量、慢性中毒对机体仍有一定的影响。

Apoptosis in mouse after tetramine poisoning

Objective To observe pathological changes and apoptosis in mouse brain,hart,liver and kidney after tetramine poisoning,and to provide some references for forensic identification.Methods An experimental model of mouse tetramine poisoning was found,and the technology of apoptosis detection was used.The staining results were analyzed by computer image analysis competitive system.Results(1)Quantities of apoptosis in brain,hart,liver and kidney in chronic poisoning groups are many more than in acute poisoning groups.As in same organs,Quantities of apoptosis are different after different poisoning time.In addition,the peak-time of apoptosis is not same in different organs.(2)Poison analysis showed that,all organic samples from acute poisoning groups were positive,while that from chronic poisoning groups are negative.Conclusion Chronic tetramine poisoning can not be detective by clinic or poison analysis while can be found changes in apoptosis,which indicate that small amount and chronic poisoning still affects the body.