| M48磁珠法和Chelex-100法提取脱落细胞DNA的初步比较 |
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| 引用本文： | 王彦涛,宋道江. M48磁珠法和Chelex-100法提取脱落细胞DNA的初步比较[J]. 刑事技术, 2013, 0(2): 53-54 |
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| 作者姓名： | 王彦涛 宋道江 |
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| 作者单位： | 湖北省十堰市公安局刑科所,442000 |
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| 摘 要： | 目的对M48磁珠法和Chelex-100法提取脱落细胞DNA的检验效果进行比较，为优化提取方法提供参考。方法选取案件受理检材50例烟蒂、50例纺织物品、50例作案工具，根据不同条件分别用吸附法、沾附法获得DNA，采用磁珠法（M48）和Chelex-100法提取后，常规STR检测。结果烟蒂类检材采用2种方法提取DNA，所得结果没有明显差异；纺织物品和作案工具上脱落细胞DNA的提取采用M48磁珠法提取的效果明显优于Chelex-100法。结论相对而言，M48磁珠法更具优势。
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| 关 键 词： | 脱落细胞DNA的提取 磁珠（M48）法 Chelex-100法 STR |
Comparison study on application of M48kit and Chelex-100 in trace DNA extraction |
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| WANG Yan-tao , SONG Dao-jiang. Comparison study on application of M48kit and Chelex-100 in trace DNA extraction[J]. Forensic Science and Technology, 2013, 0(2): 53-54 |
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| Authors: | WANG Yan-tao SONG Dao-jiang |
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| Affiliation: | (Institute of Forensic Sciences, Public Security Bureau of Shiyan City, Hubei 442000, China) |
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| Abstract: | In this paper, trace DNAs were extracted from 150 samples（50 cigarette butts, 50 textiles and 50 tools） by using M48kit and Chelex-100 respectively, and then were analyzed by 3130 genetic analyzer. The results showed that M48kit was more effective for extracting DNA from three type samples. |
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| Keywords: | DNA extract M48kit Chelex-100 |
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