浮游生物16S rDNA检测在大鼠溺死鉴定中的价值

目的建立浮游生物16S rDNA PCR检脸法应用于溺死的鉴定。方法将15只SD大鼠随机分为溺死组、死后抛尸组和正常对照组,死后分别取脑、肝、肾和肺等脏器,提取DNA.用特异性引物扩增浮游生物16S rDNA第三和第四可变区特异片段。结果溺死组5例,肺全部检出(100%)浮游生物16S rDNA;肝、肾分别检出4例(80%);脑检出3例(75%)。死后抛尸组仅1例在肺中检出浮游生物(20%)。正常对照组均未检出。苗论浮游生物16S rDNA PCR检验法可用于溺死的鉴定。

The value of plankton 16S rDNA detection on identification of drowning rat

Objective To develop a PCR-based method of detecting plankton 16S rDNA for the i dentification of death by drowning. Methods Fifteen Sprague-Dawley rats were divided randomly into three groups: the death by drowning group, the group of submerging after death and the control group. After sacrificing by different ways, the brain, liver, kidney and lung of rats were taken out respectively and DNA were extracted from the tissues of these organs and were amplified subsequently by specific primers selected from the third and fourth variable regions of plankton 16S rDNA. Results The specific amplification products were detected from all 5 samples of lung tissue ( 100% ) , 4 samples from liver and kidney tissues (80% ) , and 3 samples from brain tissue (75% ) in the group of death by drowning. No amplification product was detected in all samples of the control group and the amplification product was detected only in 1 sample of lung (20% ) in the group of submerging after death. Conclusion The PCR-based method of detecting plankton 16S rDNA for the identification of death by drowning is certainly feasible.