应用PCR检测成年鸭体内鸭瘟强毒的分布

鸭瘟病毒 (DPV)强毒经人工接种和同居感染 10 0日龄鸭后 ,应用聚合酶链反应 (PCR)检测病毒在鸭体内各组织器官的动态分布。试验结果表明 ,DPV强毒经肌肉注射进入鸭体后 6h可在肝、脾、血液和粪便中检测到DPVDNA ;DPV强毒经肌肉注射到鸭体后各受检样品被检测到DPVDNA的先后顺序为 :肝、脾、血液和直肠粪便 (6h)→肺、脑和腿肌 (12h)→肾和胸肌 (2 4h) ;同居鸭于混群后 4 8h在肝、肺、血液和直肠粪便中检出DPVDNA ;DPV强毒经同居感染鸭后各受检样品检测到DPVDNA的先后顺序为 :肝、肺、血液和直肠粪便 (48h)→脾和脑 (72h)→胸肌和腿肌 (96h)→肾 (12 0h)。

Detection of distribution of virulent duck plague virus in adult ducks by PCR

The polymerase chain reaction (PCR) technique was used to determine virulent duck plague virus (DPV) in 100 day-old ducks infected artificially with DPV and infected naturally with DPV. The (result) (revealed) that after intramuscular injection the DPV DNA was found in liver, spleen, blood, excrement, lung, brain, leg muscle, kidney and thoracic muscle from the ducks infected artificially with DPV in the virus appearing time order and that the DPV DNA was found in liver, lung, blood, excrement, spleen, brain, leg muscle, thoracic muscle and kidney from the ducks infected naturally with DPV in the virus (appearing) time order.