An ELISA using avidin-biotin complex for the determination of ABH group from saliva |
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| Authors: | T Takatori Y Tsutsubuchi |
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| Affiliation: | 1. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., Prague 166 10, Czech Republic;2. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i., Prague 142 20, Czech Republic;3. Institute of Pharmacology and Toxicology, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen 326 00, Czech Republic;1. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil;2. Departamento de Química, Centro de Ciências Físicas e Matemáticas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil;3. Departamento de Biologia Celular, Embriologia e Genética, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil;1. Division of Neurotoxicology, National Center for Toxicological Research, U.S. Food & Drug Administration, Jefferson, AR 72079, United States;2. Toxicologic Pathology Associates, Inc., Jefferson, AR 72079, United States |
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| Abstract: | The ABH group in a trace amount of saliva could be determined by an enzyme-linked immunosorbent assay (ELISA) using an avidin-biotin-peroxidase complex (ABC) technique. In this method ABH blood group substances as a solid phase are adsorbed to wells of a microtiter plate made of polystyrene. The primary antibody corresponding to the blood antigen adheres onto the wells, and reacts with the biotinylated secondary antibody. The previously formed ABC reagent is then added to the above wells, and finally the absorbance produced by the interaction of the peroxidase activity with a chromogenic substance is measured at 492 nm. This method proved to be clearly more sensitive for the detection of ABH blood groups in secretor-saliva than the conventional hemagglutination inhibition test. Also the ABH group of non-secretor-saliva could be easily determined by this method. |
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