淮海战役士兵遗骸的Y染色体遗传类型鉴定

目的鉴定淮海战役士兵遗骸的Y染色体遗传类型,为寻找其父系亲属提供线索。方法采用古DNA的方法提取遗骸DNA,使用Yfiler试剂盒进行17个Y-STR基因座的复合扩增,推测样本的单倍群,并根据最新Y染色体谱系树挑选Y-SNP位点进行精细分型,再基于Y-SNP和Y-STR数据进行共享单倍型分析,获得与遗骸遗传关系最近的现代个体信息。结果 8份男性样本中的17个Y-STR基因座总共观察到8种Y-STR单倍型,进一步Y-SNP分析得出6种Y-SNP单倍群,分别是O2a1-M95+、O1a1-P203+、O3*-M122+/M234-、D1-M15+、C3*-ST和R1a1-M17+。结论本次对淮海战役士兵遗骸进行的Y染色体遗传类型鉴定对于推断陈年检材的地理来源具有一定的借鉴价值。

Identification of Y-chromosomal Genetic Types for the Soldier's Remains from Huaihai Campaign

Objective To identify the Y-chromosomal genetic types for the soldier's remains from Huaihai Campaign,and to offer a clue for search of their paternal relatives.Methods DNA of the remains were extracted by the ancient DNA extraction method.Yfiler kit was used for the multiplex amplification of 17 Y-STR loci.The haplogroups of the samples were speculated.Detailed genotyping of the selected Y-SNP was performed based on the latest Y-chromosome phylogenetic tree.Haplotype-sharing analysis was done based on the data of Y-SNP and Y-STR,the closest modern individual information to the genetic relationship of remains was gained.Results A total of 8 Y-STR haplotypes were observed on 17 Y-STR loci of 8 male individuals.Furthermore,6 Y-SNP haplogroups were identified,which were O2a1-M95+,O1a1-P203+,O3*-M122+/M234-,D1-M15+,C3*-ST and R1a1-M17+.Conclusion Identification of Y-chromosomal genetic types for the soldier's remains from Huaihai Campaign shows a reference value on inferring the geographical origins of old materials.