血液中乙醇检验全自动体系的建立

目的建立血液中乙醇含量检验的自动化体系。方法采用自动化工作站提取-顶空气相色谱检验。在自动化工作站提取中,对负压采血、顶空瓶密封时间、取样针进行考察优化,并对自动取样和手工取样进行比较。结果乙醇的自动化工作站提取-顶空气相色谱检验的定量分析数据稳定,两份平行样品相对相差小于5%,优于手工提取。乙醇含量在0.1~3.0 mg/m L范围内线性关系良好(r≥0.999),重现性好。结论该方法具有操作简单快捷、实验流程更加规范、实验数据更加准确、消除人工操作误差、重现性好等特点,可广泛应用于血液中乙醇定性定量的检验鉴定。

Establishment of Automation System for Detection of Alcohol in Blood

Objective T o establish an autom ation system for detection of alcohol content in blood. Meth-ods T he determ ination w as perform ed by autom ated w orkstation of extraction-headspace gas chrom atog-raphy (H S-G C ). T he blood collection w ith negative pressure, sealing tim e of headspace bottle and sam-ple needle w ere checked and optim ized in the abstraction of autom ation system . T he autom atic sam pling w as com pared w ith the m anual sam pling. Results T he quantitative data obtained by the autom ated w ork-station of extraction-H S-G C for alcohol w as stable. T he relative differences of tw o parallel sam ples w ere less than 5%. T he autom ated extraction w as superior to the m anual extraction. A good linear rela-tionship w as obtained at the alcohol concentration range of 0.1-3.0 m g/m L (r≥0.999) w ith good re-peatability. Conclusion T he m ethod is sim ple and quick, w ith m ore standard experim ent process and ac-curate experim ental data. It elim inates the error from the experim enter and has good repeatability, w hich can be applied to the qualitative and quantitative detections of alcohol in blood.