| 利用AutoMate Express~(TM)系统提取陈旧性骨骼DNA |
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| 引用本文： | 李斌,吕政. 利用AutoMate Express~(TM)系统提取陈旧性骨骼DNA[J]. 法医学杂志, 2017, 33(4). DOI: 10.3969/j.issn.1004-5619.2017.04.010 |
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| 作者姓名： | 李斌 吕政 |
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| 作者单位： | 1. 福建省公安厅刑事技术总队,福建福州,350003;2. 福州市鼓楼区公安局,福建福州,350003 |
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| 基金项目： | 福建省自然科学基金资助项目 |
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| 摘 要： | 目的建立利用AutoMate Express~(TM)系统提取陈旧性骨骼DNA的方法。方法将骨骼用冷冻研磨机研磨成骨粉,经AutoMate Express~(TM)系统提取后,用Identifiler~Plus、MiniFiler~(TM)试剂盒扩增分型。结果10例保存在不同环境中、死亡时间在10~20年的骨骼样本利用AutoMate Express~(TM)系统3 h内完成DNA提取,有8例获得完整STR分型。结论 AutoMate Express~(TM)系统能快速、高效地提取陈旧性骨骼DNA,可应用于法医实际案件检验。
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| 关 键 词： | 法医遗传学 骨骼 DNA AutoMate ExpressTM系统 |
DNA Extraction from Old Bones by AutoMate ExpressTM System |
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| LI Bin,L Zheng. DNA Extraction from Old Bones by AutoMate ExpressTM System[J]. Journal of Forensic Medicine, 2017, 33(4). DOI: 10.3969/j.issn.1004-5619.2017.04.010 |
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| Authors: | LI Bin L Zheng |
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| Affiliation: | LI Bin,L(U) Zheng |
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| Abstract: | Objective To establish a method for extracting DNA from old bones by AutoMate ExpressTM system.Methods Bones were grinded into powder by freeze-mill.After extraction by AutoMate ExpressTM,DNA were amplified and genotyped by Identifiler(R) Plus and MinFilerTM kits.Results DNA were extracted from 10 old bone samples,which kept in different environments with the postmortem interval from 10 to 20 years,in 3 hours by AutoMate ExpressTM system.Complete STR typing results were obtained from 8 samples.Conclusion AutoMate ExpressTM system can quickly and efficiently extract DNA from old bones,which can be applied in forensic practice. |
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| Keywords: | forensic genetics bone DNA AutoMate ExpressTM system |
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