| 野生鸡枞菌多糖的提取及其免疫活性分析 |
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| 引用本文： | 王思芦,汪开毓,彭艳伶. 野生鸡枞菌多糖的提取及其免疫活性分析[J]. 中国兽医科学, 2011, 0(12) |
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| 作者姓名： | 王思芦 汪开毓 彭艳伶 |
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| 作者单位： | 四川农业大学动物医学院;西昌学院动物科学学院;动物疫病与人类健康四川省重点实验室;西昌市畜牧局; |
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| 基金项目： | 四川省教育厅资助科研项目(09ZB077); 教育部“长江学者和创新团队发展计划”创新团队项目(IRT0848) |
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| 摘 要： | 采用水提醇沉法获得鸡枞菌多糖,并对其总糖含量进行了测定;研究了不同剂量的鸡枞菌多糖对正常小鼠的免疫器官指数、血清溶血素水平、腹腔巨噬细胞吞噬功能及脾淋巴细胞增殖等免疫指标的影响。结果:鸡枞菌多糖得率为6.27%,总糖含量为94%;根据体重腹腔注射20mg/kg的鸡枞菌多糖可明显提高正常小鼠的溶血素水平(P<0.01)、脾指数(P<0.01)、胸腺指数(P<0.05)和巨噬细胞吞噬功能(P<0.01);体外试验中,与空白对照组相比,25μg/mL的鸡枞菌多糖可促进体外脾淋巴细胞增殖(P<0.05),12.5μg/mL鸡枞菌多糖对ConA或LPS引起的脾淋巴细胞增殖均有显著的协同作用(P<0.01)。结果表明,鸡枞菌多糖能显著增强小鼠的体液免疫和细胞免疫功能,有望成为一种新的天然免疫增强剂。
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| 关 键 词： | 鸡枞菌多糖 免疫器官指数 溶血素 巨噬细胞吞噬功能 淋巴细胞增殖 |
Immune activity of a polysaccharide isolated from wild Collybia albuminosa |
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| WANG Si-lu,,WANG Kai-yu,,PENG Yan-ling. Immune activity of a polysaccharide isolated from wild Collybia albuminosa[J]. Chinese Veterinary Science, 2011, 0(12) |
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| Authors: | WANG Si-lu WANG Kai-yu PENG Yan-ling |
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| Affiliation: | WANG Si-lu1,2,WANG Kai-yu1,3,PENG Yan-ling4(1.College of Veterinary Medicine,Sichuan Agricultural University,Ya'an 625014,China,2.College of Animal Science,Xichang College,Xichang 615013,3.Key Laboratory of Animal Disease and Human Health of Sichuan Province,4.Xichang Animal Husbandry and Veterinary Bureau,Xichang 615000,China) |
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| Abstract: | Collybia albuminosa polysaccharide(CAP)was purified by water extraction and alcohol precipitation,and its total sugar content was detected by phenol-sulphuric acid method.The effects of CAP on immune organ indices,blood serum hemolysin level,peritoneal macrophage phagocytosis function and splenic lymphocyte proliferation were detected in mice treated with CAP by peritoneal injection.The results indicated that the yield of CAP was 6.27% and the content of its total sugar was 94%.Compared with control,CAP at ... |
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| Keywords: | Collybia albuminosa polysaccharides immune organ indices serum hemolysin peritoneal macrophage phagocytosis function splenic lymphocyte proliferation |
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