高效液相色谱法测定血浆中雷公藤甲素和雷公藤酮 |
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引用本文: | 王朝虹,张吉林,何毅,侯小平. 高效液相色谱法测定血浆中雷公藤甲素和雷公藤酮[J]. 中国法医学杂志, 2004, 19(5): 268-270 |
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作者姓名: | 王朝虹 张吉林 何毅 侯小平 |
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作者单位: | 1. 公安部物证鉴定中心,北京,100038 2. 黑龙江省公安厅,哈尔滨,150008 |
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摘 要: | 目的 建立高效液相色谱法定量检测人血浆中雷公藤甲素和雷公藤酮的分析方法。方法 以Oasis HLB固相萃取柱对样品进行提取 ,应用HPLC色谱法二极管阵列检测器测定。结果 该方法的回收率高于 80 % ,线性范围在 10~ 10 0 0ng/ml ,经该方法测得雷公藤甲素的最小检出限为 3 0ng/ml,雷公藤酮的最小检出限为 4 5ng/ml(S/N≥ 3 )。结论 该方法快速灵敏、准确 ,适用于雷公藤中毒的法医学检验。
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关 键 词: | 法医毒物分析 雷公藤甲素 雷公藤酮 高效液相 固相萃取 |
文章编号: | 1001-5728(2004)05-0268-03 |
修稿时间: | 2003-12-01 |
Determination of Triptolide and Triptonide in plasma by HPLC |
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Abstract: | Objective To establish a method for quantitative determination of triptolide and triptonide in human plasma samples by HPLC.Methods The samples were extracted with Oasis HLB solid phase extraction cartridge and analyzed with reserved phase HPLC coupled with diode array detection.Results Recoveries rates obtained from spiked plasma for the two diterpenoid were higher than 80%.The linearity ranged from 10 ng/ml to 1 000 ng/ml.Detection limits of the method were 3.0ng/ml for triptolide and 4.5ng/ml for triptonide.Conclusion The method is fast and accurate,which is applicable to determination of diterpenoid in cases of suspected poisoning of Triptergium wilfordii Hook.f. in forensic practice. |
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Keywords: | Forensic toxicological analysis Triptolide Triptonide HPLC SPE |
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