Analysis of amphetamines by supercritical fluid chromatography,high-performance liquid chromatography,gas chromatography and capillary zone electrophoresis; a preliminary comparison |
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| Affiliation: | 1. Istituto Zooprofilattico Sperimentale della Lombardia e dell''Emilia Romagna “Bruno Ubertini”, Via Antonio Bianchi 7/9, 25124 Brescia, Italy;2. INSTM & Chemistry for Technologies Laboratory, Department of Mechanical and Industrial Engineering, University of Brescia, Via Branze 38, 25123 Brescia, Italy;1. Laboratoire des Sciences de l''Environnement Marin LEMAR-UMR6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Place Copernic, Technopôle Brest-Iroise, 29280 Plouzané, France;2. Ifremer, Station de Biologie Marine, Place de la Croix, 29185 Concarneau, France;3. Ifremer, Dyneco/Pelagos, ZI de la Pointe du Diable, CS10070, 29280 Plouzané, France |
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| Abstract: | A supercritical fluid chromatographic method with UV detection (SFC–UV) for the quantitative separation of phenylisothiocyanate (PITC)-derivatised amphetamines is described and compared to high-performance liquid chromatography–diode array detection (HPLC–DAD), gas chromatography–flame ionisation detection (GC–FID) and capillary zone electrophoresis–diode array detection (CZE–DAD) analyses of amphetamine and related compounds. Difficulties in the analysis of common amphetamines by SFC are discussed. Of the methods described in this paper, the SFC method offered the greatest sensitivity at 0.01–0.02 μg of drug on column. Suggestions are made for the use of combinations of these techniques for the identification of amphetamines when gas chromatography–mass spectrometry is not available. |
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