| 实验性早期心肌缺血结蛋白的免疫组织化学研究 |
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| 引用本文： | 胡丙杰,陈玉川,祝家镇,陈建国,毕启明,李杰. 实验性早期心肌缺血结蛋白的免疫组织化学研究[J]. 法律与医学杂志, 1998, 0(4): 148-150 |
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| 作者姓名： | 胡丙杰 陈玉川 祝家镇 陈建国 毕启明 李杰 |
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| 作者单位： | 中山医科大学法医病理学教研室!广州，510089，中山医科大学法医病理学教研室!广州，510089，中山医科大学法医病理学教研室!广州，510089，中山医科大学法医病理学教研室!广州，510089，中山医科大学法医病理学教研室!广州，510089，广东省东莞市公安局法医室!511700 |
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| 摘 要： | 目的 探讨急性心肌缺血早期不同缺血时间心肌细胞内结蛋白的变化 ,为早期心肌梗死死后诊断提供新的方法。方法 建立大鼠急性心肌缺血模型 ,用免疫组织化学S -P法研究心肌细胞内结蛋白的缺失情况。结果 正常心肌细胞结蛋白呈棕褐色阳性反应 ,急性心肌缺血 15min ,结蛋白即可在缺血区心肌呈小灶性缺染 ,随缺血时间延长 ,其缺染范围逐步扩大。结论 心肌细胞结蛋白的缺失是早期心肌缺血的极为敏感的指标 ,免疫组织化学染色法检测心肌细胞结蛋白的变化可望成为早期心肌缺血死后诊断的一种有意义的手段。
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| 关 键 词： | 早期心肌缺血 结蛋白 免疫组织化学 |
Immunohistochemical Study of Desmin in Experimental Early Myocardial Ischemia |
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| Hu Bingjie,Chen Yuchuan,Zhu Jiazhen,Chen Jianguo,Bi Qiming,Li Jie. Immunohistochemical Study of Desmin in Experimental Early Myocardial Ischemia[J]. The Journal of Law & Medicine, 1998, 0(4): 148-150 |
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| Authors: | Hu Bingjie Chen Yuchuan Zhu Jiazhen Chen Jianguo Bi Qiming Li Jie |
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| Affiliation: | Hu Bingjie1,Chen Yuchuan1,Zhu Jiazhen1,Chen Jianguo1,Bi Qiming1,Li Jie2 1Department of Forensic Pathology,Sun Yat-sen University of Medical Sciences,Guangzhou,510089,2Public Security Bureau of Dongguan City,Guangdong,511700 |
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| Abstract: | Objective To study the changes of cardiac desmin in different ischemic period,providing a new method for postmortem diagnosis of early myocardial infarctionMethods The rat model of acute myocardial ischemia was established,immunohistochemical S-P method was used to observe the depletion of desmin in cardiomyocytesResults The desmin in normal cardiomyocytes showed positive brown staining,but focal depletion of desmin could be detected in ischemic myocardium of 15 min,and the area of depletion of desmin became more severe with the elongation of ischemiaConclusion The deplection of desmin in cardiomyocytes is a quite sensitive marker of early myocardial ischemia,immunhistochemical detection of desmin in cardiomyocytes will be a meaningful tool for the postmortem diagnosis of early myocardial ischemia |
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| Keywords: | Early myocardial ischaemia Desmin Immunohistochemistry |
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