| 焦磷酸测序技术分析单核苷酸多态性在法医学中的应用 |
| |
| 引用本文： | 冯婷,李淑瑾,丛斌,娄春光. 焦磷酸测序技术分析单核苷酸多态性在法医学中的应用[J]. 中国法医学杂志, 2010, 25(5): 335-337,341 |
| |
| 作者姓名： | 冯婷 李淑瑾 丛斌 娄春光 |
| |
| 作者单位： | 河北医科大学法医学系,河北省法医学重点实验室,河北,石家庄,050017 |
| |
| 摘 要： | 单核苷酸多态性（SNP）是新一代的法医学遗传标记,有望成为法医实践中解决高度降解检材及特殊案件DNA鉴定的重要工具。近年来涌现出许多高通量的SNP分析方法,如引物延伸结合时间飞行质谱分析法、微测序法、SNP lex以及焦磷酸测序法等。本文重点对焦磷酸测序技术的原理、步骤及其在法医学中的应用进展进行简要的综述。
|
| 关 键 词： | 法医物证学 单核苷酸多态性 焦磷酸测序技术 |
SNP analysis using pyrosequencing and its forensic application |
| |
| FENG Ting,LI Shujin,CONG Bin,LOU Chunguang. SNP analysis using pyrosequencing and its forensic application[J]. Chinese Journal of Forensic Medicine, 2010, 25(5): 335-337,341 |
| |
| Authors: | FENG Ting LI Shujin CONG Bin LOU Chunguang |
| |
| Affiliation: | ( Hebei Key Laborotary of Forensic Medcine, Department of Forensic Medicine, Hebei Medical University, Shifiazhuang 050017, China) |
| |
| Abstract: | Single nucleotide polymorphism(SNP) is a new generation of genetic markers in forensic science,and is expected to be used in DNA typing of highly degraded forensic samples or solving some special DNA analysis.Many high-throughput SNP analysis techniques have emerged in recent years,including primer extension combined matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS),minisequencing,SNPlex and pyrosequencing method,etc.This article focuses on the principle and steps of pyrosequencing,as well as its application in forensic medicine. |
| |
| Keywords: | forensic biological evidence single nucleotide polymorphism pyrosequencing |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
