| 应用RAPD技术分析禽多杀性巴氏杆菌广西分离株的DNA多态性 |
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| 引用本文： | 刘加波,谢芝勋,唐小飞,邓显文,庞耀珊,谢志勤.应用RAPD技术分析禽多杀性巴氏杆菌广西分离株的DNA多态性[J].中国兽医科学,2004,34(8):32-35. |
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| 作者姓名： | 刘加波 谢芝勋 唐小飞 邓显文 庞耀珊 谢志勤 |
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| 作者单位： | 广西兽医研究所,广西,南宁,530001 |
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| 基金项目： | 广西区水产畜牧局资助项目 [桂渔牧发 (2 00 0 ) 5 2号 ] |
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| 摘 要： | 应用随机扩增多态性DNA(RAPD)技术 ,以随机引物单条或成对组合的方式对 9株禽巴氏杆菌 (PM )广西分离株和 3个参考株的DNA进行了多态性分析。结果显示 ,在选用的 2 0个引物中 ,有 4对引物 (4条引物成对组合 )扩增的条带为 1～ 16条 ,其长度在 90～ 2 6 0 0bp。相似指数分析显示 ,PM广西分离株GX2与GX4的相似指数最高 ,为 0 .97;GX7与B2 6T12 0 0的相似指数最低 ,为 0 .2 1,C4 8 1标准强毒株与PM广西分离株间的相似指数为 0 .33～ 0 .6 4。表明广西当地的流行株呈现多样性 ,与PM标准强毒株存在差异。
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| 关 键 词： | RAPD 禽多杀性巴氏杆菌 DNA多态性 |
| 文章编号： | 1000-6419(2004)08-0032-04 |
| 修稿时间： | 2004年4月12日 |
Study on DNA polymorphism of Pasteurella multocida strains from Guangxi by randomly amplified polymorphic DNA |
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| LIU Jia-bo,XIE Zhi-xun,TANG Xiao-fei,DENG Xian-wen,PANG Yao-shan,XIE Zhi-qin.Study on DNA polymorphism of Pasteurella multocida strains from Guangxi by randomly amplified polymorphic DNA[J].Veterinary Science in China,2004,34(8):32-35. |
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| Authors: | LIU Jia-bo XIE Zhi-xun TANG Xiao-fei DENG Xian-wen PANG Yao-shan XIE Zhi-qin |
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| Abstract: | The DNA polymorphisms of 9 Guangxi isolates of Pasteurella multocida (PM) and 3 reference strains were analyzed by randomly amplified polymorphic DNA(RAPD) with the paired combination of arbitrary primers. The amplification results showed that 1-16 bands were amplified with 4 pairs of primers out of 20 primers, and the length of the bands was between 90-2 600 bp.It was also showed that the highest similar indexes of GX2 to GX4, GX1 to GX2 are all 0.97, the lowest similar index of GX7 to B26-T1200 is 0.21; and the similar indexes of standard virulent strain C48-1 to Guangxi isolates are (between) 0.33 and 0.64. The results indicated that PM Guangxi isolates show polymorphism and (difference) from the standard virulent strain C48-1. |
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| Keywords: | randomly amplified polymorphic DNA(RAPD) Pasteurella multocida polymorphism |
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