屠宰猪肝和血清中乙型肝炎病毒及戊型肝炎病毒的检测

应用1对乙型肝炎病毒(HBV)S基因保守区的引物,采用PCR方法从屠宰猪肝、血清中检测到了HBV,序列分析表明,扩增片段与已发表的HBV S基因的同源性高达98%~100%。电镜负染色样品观察结果表明,在HBV表面抗原ELISA检测强阳性反应的血清样品中存在有形态、大小与人HBV Dane颗粒和小球状颗粒相似的病毒粒子。针对戊型肝炎病毒(HEV)ORF2/ORF3重叠区设计了简并引物,采用巢式RT-PCR对屠宰猪肝和血清样品进行了检测。结果表明,部分屠宰猪肝中存在HEV。

Detection of swine hepatitis B virus and E viruses in the liver and serum in pigs in China

The study reported the presence of swine hepatitis B(HBV) and E(HEV) viruses in the liver and serum in pigs in China.Two primers for S gene of swine HBV were designed and synthesized according to sequences of the S genes of human HBV in the literature.Swine liver and serum samples were collected from a slaughter house.Swine S genes were amplified with RT-PCR and sequenced.Results showed that sequences of S genes of swine and human HBV viruses shared 98%-100% homology.Emission transmission electron microscopy examination of HBV-positive serum revealed presence of virus particles 40-20 nm in diameter.These particles were similar to human HBV particles in terms of both diameters and shapes.A positive serum was defined by the presence of the HBV surface antigen using the ELISA method.Responses in a nested RT-PCR assay to a pair of degenerated primers designed from ORF2/ORF3 overlapping region indicated presence of gene sequence of HEV in the liver,but not serum,in some pigs.