HLA-A基因座多态性在亲子鉴定中的应用研究 |
| |
引用本文: | 刘利民,梁健,王保捷,丁梅,王丽慧,李建平,李春梅,刘永锋. HLA-A基因座多态性在亲子鉴定中的应用研究[J]. 中国法医学杂志, 2000, 15(1): 22-23 |
| |
作者姓名: | 刘利民 梁健 王保捷 丁梅 王丽慧 李建平 李春梅 刘永锋 |
| |
作者单位: | 1. 中国医科大学法医血清学教研室,沈阳,110001 2. 中国医科大学第一临床学院器官移植科,沈阳,110001 |
| |
基金项目: | 国家教委留学归国人员科研基金!(95135),辽宁省科委自然科学基金资助项目!(962312) |
| |
摘 要: | 首次将HLA A基因座的聚合酶链反应 寡核苷酸探针 (PCR SSOP)杂交分型技术应用于亲子鉴定案例分析 ,以获得HLA A基因座多态性用于法医学鉴定分析的基础数据。HLA A基因座基因分型的等位基因检出率 ( 2 4个 )和非父排除率 ( 73 3 % )均高于血清学检测方法 ;正常家系分析结果符合孟德尔遗传规律。对 3 9个亲子鉴定例的成员进行HLA A基因检测 ,9例排除 ( 2 3 1% ) ;未排除的 3 0例的亲子关系概率在 65 2 %~ 99 5 %之间。用PCR SSOP技术对HLA基因座基因分型不仅可以应用于亲子鉴定和法医学个人识别 ,还可以应用于器官移植配型及人类遗传学研究。
|
关 键 词: | 法庭科学 亲子鉴定 HLA-A基因座 PCR-SSOP |
修稿时间: | 1999-05-01 |
Study of HLA-A locus polymorphism for paternity testing |
| |
Abstract: | A technique based on PCR amplification and genetic typing with a set of SSO probes was used for 39 paternity testing cases at HLA class I A locus.The data for forensic analysis was obtained .The number of alleles and EPP obtained at HLA A locus by this technique were higher than that by serological method.The pedigree analysis was correct and proved in Mendelian inheritance. Out of 39 cases,9 cases were excluded (23.10%) and the range of RCP value unexcluded were between 65 2% and 99 5%. This genetic typing system for HLA A locus can be applied not only to paternity testing and individual identification but also to organ transplantation and anthropolgy. |
| |
Keywords: | Forensic sciences Paternity testing HLA-A locus PCR- SSOP |
本文献已被 CNKI 万方数据 等数据库收录! |