A quantitative densitometric determination of heroin and cocaine samples by high-performance thin-layer chromatography |
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| Authors: | E Della Casa G Martone |
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| Affiliation: | 1. Department of Research and Development, PolyCaps Ltd., Tel Aviv 61580, Israel;2. Department of Pharmaceutical Engineering, The Azrieli Jerusalem College of Engineering, Jerusalem 91035, Israel;1. Center for Health Services Research, Department of Internal Medicine, University of Kentucky,740 South Limestone Avenue, Lexington, KY, 40536, USA;2. Center for Applied Research on Substance Use and Health Disparities, Nova Southeastern University, 7255 NE 4th Avenue, Suite 112, Miami, FL, 33138, USA;3. Department of Psychiatry, Division of Child Psychiatry, Case Western Reserve University, 10524 Euclid Avenue #1155A, Cleveland, OH, 44106 USA;4. Department of Psychiatry,Washington University in St. Louis, 660 South Euclid Avenue, St. Louis, MO, 63110, USA;1. Bielefeld University, Center for Biotechnology, Universitätsstraße 27, 33615 Bielefeld, Germany;2. Federal Criminal Police Office, Forensic Science Institute, Äppelallee 45, 65203 Wiesbaden, Germany;3. Bundeswehr Institute of Microbiology, Neuherbergstraße 11, 80937 Munich, Germany;1. Immunoassay/Immunosensors Lab, Institute of Nuclear&Radiological Sciences&Technology, Energy&Safety, NCSR “Demokritos”, GR-15341 Aghia Paraskevi, Greece;2. Forensic Science Division, Hellenic Police, Athens, Greece;3. Immunopeptide chemistry Lab, Institute of Nuclear&Radiological Sciences&Technology, Energy&Safety, NCSR “Demokritos”, GR-15341 Aghia Paraskevi, Greece;4. ThetaMetrisis S.A., Polydefkous 14, GR-12243 Athens, Greece;5. Optical sensors Lab, Institute of Nanoscience and Nanotechnology, NCSR “Demokritos”, GR-15341 Aghia Paraskevi, Greece;6. Electronics Eng. Dept., TEI of Piraeus, Athens, Greece;1. Kusuma School of Biological Sciences, Indian Institute of Technology (IIT), Hauz Khas, New Delhi 110016, India;2. Dr. Reddy''s Institute of Life Sciences, University of Hyderabad Campus, Prof. C. R. Rao Road, Gachibowli, Hyderabad 500007, India;3. Department of Clinical Microbiology, School of Medicine, Trinity College, Dublin, Ireland;4. National Institute of Pathology, Safdarjung Hospital Campus, New Delhi 110029, India |
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| Abstract: | The traditional thin-layer chromatography (TLC) is a sensitive technique frequently used in chemical analysis for semiquantitative determinations. In the last years a high-performance thin-layer chromatography system (HPTLC) has been developed. It assures quantitative determinations with direct densitometric measurements of chromatographic spots in situ on the plate. Here, we wish to report a method for the drug determination in street samples, that employs a CAMAG TLC/HPTLC Scanner Photodensitometer and a CAMAG LINOMAT III for sample application. Samples of illicit drug traffic (50 samples of street heroin and 30 samples of street cocaine) are tested and the gas-liquid chromatography (GLC) and HPTLC determinations are compared. The HPTLC method has proven to be sensitive, precise and accurate for quantitative determinations of drugs in street samples. Furthermore this method may be useful in forensic chemistry to show a "fingerprint" of each sample. |
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