Development of a novel miniSTR multiplex assay for typing degraded DNA samples |
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| Authors: | Ronny Decorte Chi Fung Liu Jean-Jacques Cassiman |
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| Institution: | a Laboratory of Forensic Genetics and Molecular Archaeology, Department of Human Genetics - K.U.Leuven, Belgium
b Department of Forensic Medicine - University Hospitals Leuven, Belgium |
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| Abstract: | A multiplex PCR was developed for the analysis of the sex-determining gene Amelogenin, four conventional STR (short tandem repeat; THO1, D18S51, D21S11 and FGA) loci with a reduced amplicon size and four miniSTR loci (D1S1677, D2S441, D10S1248 and D22S1045). A concordance study in a population of 198 Belgians revealed no differences for the conventional STR loci while a sensitivity study showed a reproducible DNA profile with as low as 30 pg of input DNA. |
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| Keywords: | miniSTR Multiplex PCR Population genetics Belgian |
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