Mini-SGM multiplex in degraded samples |
| |
Authors: | Susana Camacho,Clá udia Vieira-Silva,Teresa Ribeiro,Helena Geada |
| |
Affiliation: | a Serviço de Genética e Biologia Forense, Delegação do Sul do Instituto Nacional de Medicina Legal, Portugal b Medicina Legal e Ciências Forenses, Faculdade de Medicina da Universidade de Lisboa, Portugal |
| |
Abstract: | A five Mini-SGM multiplex which encompasses TH01, FGA, D18S51, D16S539 and D2S1338, common STR markers in human identity testing, have been performed. Two cases with different biological tissues were selected to illustrate the usefulness of this technique in forensic casework. The use of routine methodology can sometimes give only a partial genetic profile or no profile at all. However, using the Mini-STR technique, a full profile was obtained for the majority of the degraded samples. We conclude that the Mini-SGM methodology is more sensible than routine methodology for degraded samples, although a full genetic profile is not obtained in all cases as results are still very much sample-dependent. This Mini-SGM multiplex can be considered a useful tool to complement conventional STR analysis in degraded samples. |
| |
Keywords: | Mini-STRs Degraded samples Mini-SGM |
本文献已被 ScienceDirect 等数据库收录! |
|