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Polymorphism of four X-chromosomal STR loci in Belarusians and Slovaks |
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| Authors: | Lidia Cybulska,Joanna Wysocka,Ewa Kapińska,Iosif S. Tsybovsky,Zuzana D?upinková |
| Affiliation: | a Department of Forensic Medicine, Medical University of Gdańsk, ul. D?bowa 23, 80-204 Gdańsk, Poland b Institute for the Study of Arts, Ethnography and Folklore, National Academy of Sciences, Minsk, Belarus c Institute of Problems of Criminology, Criminalistics and Forensic Expertise, Minsk, Belarus d Department of Anthropology, Comenius University, Bratislava, Slovakia e Department of Experimental and Applied Genetics, Institute of Preventive and Clinical Medicine, Medical University, Bratislava, Slovakia |
| Abstract: | We have investigated four X-chromosomal STR loci (a tetranucleotide repeat marker HPRTB, DXS7423 and trinucleotide repeat systems DXS101, DXS8377) in population samples of unrelated Belarusian and Slovak males (N = 180 and 116, respectively).Markers were amplified in a multiplex PCR reaction with primers labeled with fluorescent dyes 5-FAM and 5-JOE (dye set F). The separation and detection of PCR products were performed by capillary electrophoresis on a 3130 Genetic Analyzer (Applied Biosystems), using positive controls (K562 and NA9947A) and the GeneScan-500 ROX internal lane standard. Allele designation was based on comparison with the constructed allelic ladder. We performed statistical analysis estimating power of discrimination for males (PDmale). Our data was compared with European populations from Poland and Germany. The obtained data are useful in forensic practice and they contribute to creation of national X-STR databases in Belarus and Slovakia. |
| Keywords: | X-chromosome STRs Frequency data Belarusian and Slovak population |
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