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Genetic analysis of fingerprints—Could WGA or nested-PCR be alternatives to the increase of PCR cycles number? |
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| Authors: | A.M. Lagoa,T. Magalhã es,M.F. Pinheiro |
| Affiliation: | a Medical Faculty of Oporto University, Portugal b Abel Salazar Biomedical Institute of Oporto University, Portugal c National Institute of Legal Medicine, North Delegation, Portugal d Health Sciences Faculty, Fernando Pessoa University, Portugal |
| Abstract: | In this work we aimed to compare the application of increasing PCR cycle number, whole genome amplification and nested-PCR on fingerprints genetic analysis. Results were compared for correct alleles, allele dropin and allelic dropout. We concluded that increasing the number of PCR cycles is yet the best way to attain the required sensitivity. |
| Keywords: | LCN Fingerprints WGA Nested-PCR Forensic genetics |
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