Implementation of a robotized real-time PCR setup for the use of the Quantifiler™ Human DNA Quantification Kit |
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Authors: | Ralph Schwenzer Werner Pflug |
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Affiliation: | Landeskriminalamt Baden-Württemberg, Kriminaltechnisches Institut, Taubenheimstraße 85, 70372 Stuttgart, Germany |
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Abstract: | Human DNA quantification occupies a central role within the DNA analytic process of forensic casework samples as DNA quantification results have an important impact on the quality of the short tandem repeat data. Manual processing for the setup of quantification reactions can be time consuming and labor intensive. Therefore automation of quantitative real-time PCR setup was an important component of our DNA-analysis automation concept. Here we show the implementation of a robotized setup for the Quantifiler™ Human DNA Quantification Kit. |
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Keywords: | Automation DNA quantification Forensic casework samples Real-time PCR Quantifiler&trade Human DNA Quantification Kit |
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