Application of less primer method to commercial kits |
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| Authors: | M Kane M Taniguchi S Matsuyama K Nishi |
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| Institution: | a Forensic Science Laboratory, Shiga Police Headquarters, Karasaki 1-34-3, Otsu 520-0106, Japan
b Legal Medicine, Shiga University of Medical Science, Japan |
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| Abstract: | A modified PCR method is described. We examined the conditions of commercial kits including concentration of primer, amplification cycle number and annealing and extension time to obtain even PCR products as well accurate genotype analysis. In addition, we tried to arrange the ratio of primer composition in PowerPlex® 16 system to improve the loci of high molecular and add two times of Taq Gold polymerase to decrease the extension time.The advantages of less primer method are good balance among loci, reproducibility and sensitivity. |
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| Keywords: | Less primer method Multiplex PCR |
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