Challenging DNA: Assessment of a range of genotyping approaches for highly degraded forensic samples |
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Authors: | M. Fondevila,N. Naverá n,M. Cerezo,A. Rodrí guez,R. Calvo,L.M. Ferná ndez,Á . Carracedo,M.V. Lareu |
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Affiliation: | Institute of legal Medicine, University of Santiago de Compostela, Spain |
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Abstract: | It is common in forensic casework to encounter highly degraded DNA samples from a variety of sources. In this category bone and teeth samples are often the principal source of evidential material for criminal investigations or identification of long-deceased individuals. In these circumstances standard STRs are prone to fail due to their long amplicon sizes (since DNA becomes progressively more fragmented as it degrades). To successfully resolve such cases alternative markers can be used and until recently the only other tool available was mitochondrial DNA, which despite being more resistant to degradation, is much less informative. A rapidly developing approach to analyzing degraded DNA is the typing of loci from short-amplicon PCR products based on markers such as mini-STRs and autosomal SNPs. We have performed an analysis of several cases with naturally degraded DNA using established STRs plus mini-STRs and autosomal SNPs in order to make an objective comparison of the performance of each method using challenging DNA. The main aim was to establish the benefits and drawbacks of each marker set to help the practitioner choose the DNA analysis method most suited to the circumstances of each case. |
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Keywords: | STRs Degraded DNA Short-amplicon STR analysis SNP typing |
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