| 激光显微捕获口腔上皮细胞的DNA分型 |
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| 引用本文： | 顾丽华,张晨,陈连康,郑会芬,程莉,周怀谷. 激光显微捕获口腔上皮细胞的DNA分型[J]. 法医学杂志, 2006, 22(3): 196-197,203 |
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| 作者姓名： | 顾丽华 张晨 陈连康 郑会芬 程莉 周怀谷 |
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| 作者单位： | 上海市刑事科学技术研究所,上海,200083 |
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| 摘 要： | 目的探索激光显微技术(lasercapturemicrodissectionsystem,LCM)捕获口腔上皮细胞,并进行STR-DNA分型检测的方法。方法用VERITAS显微切割仪红外低能激光显微捕获一定数量口腔上皮细胞,进行ProfilerPlus试剂盒STR复合扩增,检测DNA基因型。结果7～8个口腔上皮细胞能成功获得STR-DNA分型。3～4个口腔上皮细胞不能成功获得STR-DNA分型。结论激光显微捕获作为一种分离单个细胞的新技术,对于微量口腔上皮细胞的STR-DNA分型是可行的。
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| 关 键 词： | 激光显微捕获(LCM) 口腔上皮细胞 STR-DNA分型 |
| 文章编号： | 1004-5619(2006)03-0196-03 |
| 修稿时间： | 2006-03-31 |
DNA Genotyping of Oral Epithelial Cells by Laser Capure Microdissection |
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| GU Li-hua,ZHANG Cheng,CHEN Lian-kang,ZHEN Hui-fen,CHENG Li,ZHOU Huai-gu. DNA Genotyping of Oral Epithelial Cells by Laser Capure Microdissection[J]. Journal of Forensic Medicine, 2006, 22(3): 196-197,203 |
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| Authors: | GU Li-hua ZHANG Cheng CHEN Lian-kang ZHEN Hui-fen CHENG Li ZHOU Huai-gu |
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| Affiliation: | Shanghai Institute of Forensic Science, Shanghai, 200083, China. |
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| Abstract: | objective The STR genotypping of trace oral epithelial cells which are microdissected by laser capure microdissection system(LCM) is explored. Methods The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures. Results DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed. Conclusion It is viable in genotyping of trace oral epithelial cells by Laser Capure Microdissection as a new technology of seperating single cell. |
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| Keywords: | laser capure microdissection(LCM) oral epithelial cells STR-DNA typing |
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