STR基因座三带型等位基因的统计学分析

目的探讨STR基因座三带型等位基因的统计学方法。方法对8846份无关个体的静脉血或血痕样本利用磁珠法提取血液DNA,通过复合荧光扩增和毛细管电泳得到STR基因型,采用GeneMapperIDv3．2软件分析STR基因型,并通过直接计数法检测三带型基因型频率,公式计算三带型等位基因频率,并推导三带型统计学分析在亲缘鉴定及个体识别中的公式。结果8846份样本中,共检出4例三带型等位基因和3种三带型基因型。且发现等位基因基因频率的乘积与实际发生率差异具有统计学意义,并成功推导出三带型在亲缘鉴定及个体识别中的公式。结论三带型等位基因某两个等住基因作为整体在群体中遗传的频率可用这两个等位基因频率的乘积进行估算。

Statistics Analysis of Tri-allelic Patterns in STR loci

Objective To investigate the statistical method of tri-allelic patterns of STR loci. Methods The DNAs of venous blood and blood stain samples from 8 846 unrelated individuals were extracted us- ing magnetic bead method, and STR genotypes were determined by multiplex fluorescent amplification and capillary electrophoresis and analyzed using GeneMapper ID v3.2 software. The genotype frequency and allele frequency of trizonal were determined by direct counting and formula, respectively, in order to de- duce the formula of trizonal in paternity test and individual recognition. Results Of the 8 846 individu- als, four tri-alleles and three tri-genotypes were detected. The multiplying of allele frequency and actual rate showed significantly statistical difference. The formula of trizonal in paternity test and individual recog- nition was successfully deduced. Conclusion The frequency of the two alleles inherited as a whole in the population could be calculated by multiplying the frequencies of each allele in the tri-allelic patterns.