| 测序方法验证12个STR基因座的基因型 |
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| 引用本文： | 李成涛,赵珍敏,柳燕,李莉. 测序方法验证12个STR基因座的基因型[J]. 法医学杂志, 2009, 25(2): 115-117,122. DOI: 10.3969/j.issn.1004-5619.2009.02.011 |
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| 作者姓名： | 李成涛 赵珍敏 柳燕 李莉 |
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| 作者单位： | 1. 司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063;复旦大学生命科学学院,上海200433
2. 司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海,200063 |
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| 基金项目： | 中央级科研院所社会公益研究项目 |
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| 摘 要： | 目的 用测序方法验证12个STR基因座的基因型.方法 根据各STR基因座的特异性序列,对CSF1PO、FGA、TH01、TPOX、VWA、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51和D21S11 12个STR基因座设计了PCR引物并对标准品9947A和突变样本进行PCR产物测序.结果 标准品9947A和突变样本的测序结果均与其基因分型结果一致.结论 建立的测序方法准确、灵敏,可以用于STR基因型的确认.
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| 关 键 词： | 法医遗传学 短串联重复序列 突变 |
Genotype Validation of 12 STR Loci by Sequencing Method |
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| LI Cheng-tao,ZHAO Zhen-min,LIU Yan,LI Li. Genotype Validation of 12 STR Loci by Sequencing Method[J]. Journal of Forensic Medicine, 2009, 25(2): 115-117,122. DOI: 10.3969/j.issn.1004-5619.2009.02.011 |
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| Authors: | LI Cheng-tao ZHAO Zhen-min LIU Yan LI Li |
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| Affiliation: | LI Cheng-tao1,2,ZHAO Zhen-min1,LIU Yan1,LI Li1 (1. Shanghai Key Laboratory of Forensic Medicine,Institute of Forensic Science,Ministry of Justice,P.R. China,Shanghai 200063,China,2. School of Life Sciences,Fudan University,Shanghai 200433,China) |
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| Abstract: | Objective To validate the genotype of 12 STR loci by sequencing method. Methods According to the special sequence of 12 STR loci(CSF1PO, FGA, TH01, TPOX, VWA, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11), the relative PCR primers were designed. PCR products of control DNA(9947A) and STR mutation samples were sequenced. Results The sequencing results of both the control DNA(9947A) and STR mutation samples were consistent with their genotyping results. Conclusion The sequencing method develop... |
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| Keywords: | forensic genetics short tandem repeat mutation |
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