| 非洲马瘟病毒VP7基因在重组杆状病毒中的表达及抗原性检测 |
| |
| 引用本文： | 曹琛福,花群义,吕建强,秦智锋,杨俊兴,曾少灵,阮周曦,陈兵,史卫军. 非洲马瘟病毒VP7基因在重组杆状病毒中的表达及抗原性检测[J]. 中国兽医科学, 2010, 40(8) |
| |
| 作者姓名： | 曹琛福 花群义 吕建强 秦智锋 杨俊兴 曾少灵 阮周曦 陈兵 史卫军 |
| |
| 作者单位： | 曹琛福,花群义,吕建强,秦智锋,杨俊兴,曾少灵,阮周曦,陈兵(深圳出入境检验检疫局动植物检验检疫技术中心,广东,深圳,518010;深圳市外来有害生物检测技术研发重点实验室,广东,深圳,518010);史卫军(宝安出入境检验检疫局,广东,深圳,518010) |
| |
| 基金项目： | 国家高技术研究发展计划(863)项目，深圳出入境检验检疫局科技项目 |
| |
| 摘 要： | 利用pFastBacHTA质粒将非洲马瘟病毒(AHSV)血清4型的VP7基因重组入杆状病毒,并感染昆虫细胞Sf9。结果显示,VP7蛋白在真核细胞内获得了表达,但表达量较低。免疫印迹试验证实,VP7重组蛋白具有较好的免疫原性,将其作为检测抗原包被酶标板,初步建立了检测AHSV的间接酶联免疫吸附试验方法。
|
| 关 键 词： | 非洲马瘟病毒 VP7蛋白 真核表达 抗原性 |
Expression of VP7 gene of African horse sickness virus in recombinant baculovirus and detection of antigenicity of the expressed protein |
| |
| Affiliation: | CAO Chen-fu1,2,HUA Qun-yi1,Lü Jian-qiang1,QIN Zhi-feng1,YANG Jun-xing1,ZENG Shao-ling1,RUAN Zhou-xi1,CHEN Bing1,SHI Wei-jun3(1.Inspection & Quarantine Center for Animals and Plants,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen 518010,China,2.Shenzhen Key Laboratory of Inspection Research & Development of Alien Pests,3.Baoan Entry-Exit Inspection and Quarantine Bureau,China) |
| |
| Abstract: | In order to obtain the VP7 protein,which has the same or similar antigenicity as that of the virus,the plasmid of pFastBac HT A with AHSV-4 serotype VP7 gene was recombined into the baculovi-rus,and infected insect cells Sf9.The VP7 protein was expressed within eukaryotic cells,but the expression level was low.Western-blotting and indirect ELISA showed that the expressed VP7 protein had good immunogenicity,and could be used as antigen for clinical detection of African horse sickness.Using the expressed VP7 ... |
| |
| Keywords: | African horse sickness virus VP7 protein eukaryotic expression antigenicity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
