| 五氟丙酰衍生化-气相色谱/质谱联用法分析尿液中7-氨基硝西泮 |
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| 引用本文： | 卓晓聪,谢瑜,褚建新. 五氟丙酰衍生化-气相色谱/质谱联用法分析尿液中7-氨基硝西泮[J]. 法医学杂志, 2007, 23(3): 199-200 |
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| 作者姓名： | 卓晓聪 谢瑜 褚建新 |
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| 作者单位： | 1. 杭州市萧山区公安分局,浙江,杭州,311200
2. 浙江省人民检察院,浙江,杭州,310012 |
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| 摘 要： | 目的用五氟丙酰衍生化-气相色谱/质谱联用法分析尿液中硝西泮的主要代谢物7-氨基硝西泮。方法尿液经乙醚萃取后,用五氟丙酰酸酐(PFPA)进行衍生化,得到7-氨基硝西泮衍生化物的总离子流色谱图和质谱图。结果根据7-氨基硝西泮衍生化物色谱的保留时间和质谱中主要特征离子的质荷比进行定性分析;用7-氨基氟硝西泮做内标,以7-氨基硝西泮和内标衍生化物定量离子的色谱峰面积比与浓度的关系进行定量分析。结论用五氟丙酰衍生化-GC/MS分析尿液中硝西泮的主要代谢物7-氨基硝西泮的方法定性、定量灵敏准确,测定结果可应用于司法鉴定。
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| 关 键 词： | 气相色谱-质谱联用法 五氟丙酰衍生化 尿液 硝西泮 7-氨基硝西泮 |
| 文章编号： | 1004-5619(2007)03-0199-02 |
| 修稿时间： | 2006-02-28 |
Analysis of 7-Aminonitrazepam in Urine by Pentafluoropropionyl Derivatization- Gas Chromatography/Mass Spectrometry |
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| ZHUO Xiao-cong,XIE Yu,CHU Jian-xin. Analysis of 7-Aminonitrazepam in Urine by Pentafluoropropionyl Derivatization- Gas Chromatography/Mass Spectrometry[J]. Journal of Forensic Medicine, 2007, 23(3): 199-200 |
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| Authors: | ZHUO Xiao-cong XIE Yu CHU Jian-xin |
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| Abstract: | Objective This paper reported a new method for the analysis of 7-amnonitrazepam(7-ANIZ), the major metabolite of nitrazepam in urine by pentafluoropropionyl derivatization-GC/MS. Methods After urine sample were extracted with ethyl ether, the extracts were derivatized with pentafluoropropionic anhydride (PFPA), and then total ion current chromatograms(TIC) of the derivatives were acquired. Results The qualitative analysis of 7-ANIZ can be built by tR and m/z; The analysis of 7-ANIZ can be built by internal standard method. Conclusion The analytical method of qualitative and quantitative analysis of 7-ANIZ was sensitive and accurate. |
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| Keywords: | GC/MS pentafluoro-propionyl derivatization urine nitrazepam 7-amnonitrazepam |
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