| 新藤黄酸体外抑制肺腺癌A549细胞增殖的作用 |
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| 作者姓名： | 朱国旗 程卉 王训翠 王效山 李庆林 |
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| 作者单位： | 安徽中医学院科研实验中心,安徽,合肥,230038;安徽中医学院科研实验中心,安徽,合肥,230038;安徽中医学院科研实验中心,安徽,合肥,230038;安徽中医学院科研实验中心,安徽,合肥,230038;安徽中医学院科研实验中心,安徽,合肥,230038 |
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| 基金项目： | 安徽省高校自然科学研究项目，国家重大新药创制专项 |
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| 摘 要： | 目的 研究新藤黄酸(Gambogenic acid,GNA)抑制肺腺癌细胞株A549增殖的作用及其相关机制.方法 不同浓度GNA分别作用A549细胞24、48和72 h,甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)比色法检测药物对A549细胞增殖的抑制率,吖啶橙/溴化乙啶(acri...
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| 关 键 词： | 新藤黄酸 肺腺癌A549细胞 细胞凋亡 |
Inhibitive Effects of Gambogenic Acid on Proliferation of Lung Adenocarcinoma A549 Cells In Vitro |
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| Authors: | ZHU Guo-qi CHENG Hui WANG Xun-cui WANG Xiao-shan LI Qing-lin |
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| Institution: | ZHU Guo-qi,CHENG Hui,WANG Xun-cui,WANG Xiao-shan,LI Qing-lin(Experimental Center for Scientific Research,Anhui College of Traditional Chinese Medicine,Anhui Hefei 230038,China) |
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| Abstract: | Objective To investigate anti-proliferation effect of Gambogenic acid(GNA) on lung adenocarcinoma A549 cells and its underlying mechanisms.Methods A549 cells were treated with different concentration of GNA for 24,48 and 72 h,respectively;the inhibitive effect was measured by methyl thiazolyl tetrazolium(MTT) assay;alternation of ultra structure was detected by AO/EB staining under fluorescent microscope;lactate dehydrogenase(LDH) assay was applied to determine whether GNA induced A549 cells necrosis.Result... |
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| Keywords: | Gambogenic acid lung adenocarcinoma A549 apoptosis |
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