Whole Plastome Sequences of Two Drug-Type Cannabis: Insights Into the Use of Plastid in Forensic Analyses |
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| Authors: | Cristiane Barbosa D'Oliveira Matielo M.S. Rafael Plá Matielo Lemos Ph.D. Deise Schröder Sarzi M.S. Lilian de Oliveira Machado Ph.D. Dalvan Carlos Beise Bac. Priscila Caroline Thiago Dobbler M.S. Renata Machado Castro Bac. Mauro Sander Fett Ph.D. Luiz Fernando Würdig Roesch Ph.D. Flávio Anastácio de Oliveira Camargo Ph.D. Valdir Marcos Stefenon Ph.D. |
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| Affiliation: | 1. Núcleo de Ecologia Molecular e Micropropagação de Plantas, Universidade Federal do Pampa - UNIPAMPA, São Gabriel, 97307-020 Rio Grande do Sul, Brasil;2. Instituto de Bioquímica Médica Leopoldo de Meis - CCS, Universidade Federal do Rio de Janeiro - UFRJ, Rio de Janeiro, 21941-902 RJ, Brasil;3. Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Universidade Federal de Santa Catarina – UFSC, Florianópolis, 88040-900 Santa Catarina, Brasil;4. Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa - UNIPAMPA, São Gabriel, 97307-020 Rio Grande do Sul, Brasil;5. Setor Técnico-Científico, Superintendência Regional do Departamento de Polícia Federal no Rio Grande do Sul, Porto Alegre, 90160-093 Rio Grande do Sul, Brasil;6. Departamento de Ciências do Solo, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, 91540-000 Rio Grande do Sul, Brasil |
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| Abstract: | DNA is one of the fastest growing tools in forensic sciences, increasing reliability in forensic reports and judgments. The use of DNA has increased in different areas of the forensic sciences, such as investigation of plant species, where plastid DNA has been used to elucidate and generate evidence in cases of traceability of genetically modified and controlled plants. Even with several advances and the practice of using DNA in forensic investigations, there are just few studies related to the identification of genetic tools for the characterization of drug and nondrug-types of Cannabis. Herein, the whole plastomes of two drug-type Cannabis are presented and have their structures compared with other Cannabis plastomes deposited in the GenBank, focusing in the forensic use of plastome sequences. The plastomes of Cannabis sativa “Brazuka” and of the hybrid Cannabis AK Royal Automatic presented general structure that does not differs from the reported for other C. sativa cultivars. A phylogenomic analyses grouped C. sativa “Brazuka” with the nondrug C. sativa cultivars, while the hybrid Cannabis AK Royal Automatic placed isolated, basal to this group. This suggests that the analysis of plastomes is useful toward genetic identification of hybrids in relation to C. sativa. |
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| Keywords: | forensic science forensic genetics CPDNA hemp marijuana chloroplast genome next generation sequencing |
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