| 免疫组织化学检测MMP-11鉴定月经血 |
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| 作者姓名： | Yao YN Lu HL Chen S Zheng J Zhang YQ |
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| 作者单位： | 1. 中山大学,中山医学院法医物证学教研室,广东,广州,510080;中山大学,附属第一医院,广东,广州,510080
2. 中山大学,中山医学院法医物证学教研室,广东,广州,510080 |
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| 摘 要： | 目的建立用自制兔抗人基质金属蛋白酶-11（matrix metalloproteinase-11,MMP-11）多克隆抗体鉴定月经血的免疫组化方法,探讨MMP-11蛋白酶的细胞定位。方法运用链霉卵白素-碱性磷酸酶（SAP）免疫组化法检测人月经血及外周静脉血涂片、阴道液涂片、子宫内膜石蜡切片和陈旧月经血痕涂片中的MMP-11。结果MMP-11存在于子宫内膜上皮细胞和间质细胞内;外周静脉血和阴道液涂片未见有MMP-11染色;陈旧月经血痕涂片罕见完整子宫内膜细胞,但能检测到微弱特异信号。结论用自制抗MMP-11多克隆抗体采用免疫组化技术能有效地检测MMP-11,区分月经血和静脉血。
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| 关 键 词： | 免疫组织化学 多克隆抗体 MMP-11 月经血 |
| 文章编号： | 1004-5619(2008)01-0032-02 |
| 修稿时间： | 2007年8月22日 |
Detection of MMP-11 from menstrual blood using immunohistochemistry |
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| Yao YN,Lu HL,Chen S,Zheng J,Zhang YQ.Detection of MMP-11 from menstrual blood using immunohistochemistry[J].Journal of Forensic Medicine,2008,24(1):32-33,F0003. |
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| Authors: | Yao Ya-Nan Lu Hui-Ling Chen Sen Zheng Jing Zhang Ya-Qing |
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| Institution: | Department of Forensic Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China. |
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| Abstract: | OBJECTIVE: To prove the feasibility of detecting menstrual blood as well as its cellular localization with rabbit-anti-human matrix metalloproteinase-11 (MMP-11) polyclonal antibody. METHODS: MMP-11 in menstrual blood, peripheral blood, vaginal liquid, aged menstrual bloodstain, and endometrium sections were assayed with SAP immunohistochemistry. RESULTS: MMP-11 was found only in menstrual samples within stroma and epithelium cells. CONCLUSION: MMP-11 polyclonal antibody may be applied in the distinction between menstrual blood and venous blood. |
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| Keywords: | immunohistochemistry polyclonal antibodies MMP-11 menstrual blood |
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