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A comparison of methods used in the UK and Ireland for the extraction and detection of semen on swabs and cloth samples |
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| Authors: | JE Allard A Baird G Davidson S Jones J Lewis L McKenna C Weston D Scrimger G Teppett |
| Institution: | aForensic Science Service, 109 Lambeth Road, London SE1 7LP, United Kingdom bForensic Science Northern Ireland, 151 Belfast Road, Carrickfergus, Co. Antrim, BT38 8PL, Northern Ireland cLGC Forensics Alliance, Birchwood Park, Darwin House, Faraday Road, Risley, Warrington, WA3 6AT, United Kingdom dGrampian Police Forensic Laboratory, Nelson Street, Aberdeen, AB24 5EQ, United Kingdom eLGC Forensics Alliance, Culham Science Centre, Abingdon, Oxfordshire, OX14 3ED, United Kingdom fForensic Science Laboratory, Garda Headquarters, Phoenix Park, Dublin 8, Ireland gStrathclyde Police Forensic Support Department, Police HQ, 173 Pitt St, Glasgow G2 4JS, United Kingdom hLothian and Borders Police Forensic Science Laboratory, 11 Howden Hall Road, Edinburgh, EH16 6TL, United Kingdom iPolice Forensic Science Laboratory Dundee Tayside Police HQ, West Bell St, Dundee DD1 9JU, United Kingdom |
| Abstract: | The recent formation of a United Kingdom and Irish working group, the Body Fluids Forum (BFF), highlighted the need to investigate different working practices prior to any inter-laboratory comparison work and identification of best practice. Various dilutions of semen were seeded onto swabs and cloth samples for each BFF member laboratory to test using their standard techniques. The results showed that the detection of acid phosphatase on swabs is best achieved using direct testing rather than on an extract from the swab. Extraction methods for spermatozoa require a balance to be achieved between using a sufficient volume of water to ensure optimal release and minimal volume to ensure a concentrated extract. PSA tests were investigated and found to be more sensitive than Choline. DNA profiles were obtained from samples in which no spermatozoa had been detected during microscopic examination. |
| Keywords: | Forensic science Semen Extraction Swab Cloth Body Fluid Forum |
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