保存温度与时间对生物样品中氯胺酮稳定性的影响

目的研究不同温度和时间条件保存时生物样品中氯胺酮的稳定性。方法家兔以氯胺酮150mg/kg灌胃,30min后处死,取其血、肝、肾、脑,分别在室温（18～24℃）和冷冻（-20℃）条件下保存,并用气相色谱-质谱法定性分析、气相色谱-氮磷检测器法测定不同时间各样品中氯胺酮含量。结果血、肝、肾、脑冷冻保存至第30天氯胺酮含量均降低（P〈0.05）;室温条件下各样品中氯胺酮含量自第5天起均升高（P〈0.05）。结论生物样品在冷冻条件下保存时氯胺酮稳定性较好,怀疑氯胺酮中毒或死亡的检材应冷冻保存,尽快检测。

Effect of Temperature and Time on Stability of Ketamine in Biological Samples

Objective The stability of ketamine in biological samples was studied under different storage temperature and time. Methods The rabbits were given intragastric administration of ketamine with a dose of 150 mg/kg and were sacrificed after 30 minutes. Blood, liver, kidney and brain of the rabbits were stored at room temperature （between 18 ℃ and 24 ℃） and -20 ℃. The specimens were analyzed at different times by GC-MS and GC-NPD. Results At -20 ℃, the concentration of ketamine decreased in all of samples （P〈0.05） within 30 days. The concentration of ketamine increased in all of samples stored at room temperature after 5 days（P〈0.05）. Conclusion The stability of ketamine in biological samples stored at-20 ℃ was better than that at room temperature. The samples suspected containing ketamine should be stored at-20 ℃ and should be tested as soon as possible.