| 心肌缺血内皮素RT-PCR初步研究及其意义 |
| |
| 引用本文： | Ma MY,Xu XH. 心肌缺血内皮素RT-PCR初步研究及其意义[J]. 法医学杂志, 2005, 21(1): 21-23,26 |
| |
| 作者姓名： | Ma MY Xu XH |
| |
| 作者单位： | 1. 深圳市公安局刑科所,广东,深圳,518001
2. 汕头大学医学院,广东,汕头,515031 |
| |
| 摘 要： | 目的为探讨心肌缺血后内皮素基因在心肌组织内表达的变化。方法用RT-PCR法研究了心肌缺血60min后心肌内皮素的表达情况。结果发现RT-PCR结果显示缺血组和正常组均检出ET-1mRNA,但是二者之间有显著性差异,说明缺血可以诱导ET-1mRNA增高。结论本实验为ET-1免疫组织化学法研究奠定了分子基础。
|
| 关 键 词： | 心肌缺血 内皮素 RT-PCR 大鼠 |
| 文章编号： | 1004-5619(2005)01-0021-03 |
| 修稿时间： | 2004-02-26 |
Primarily study and significance of ET-1 during ischemia with RT-PCR |
| |
| Ma Meng-yun,Xu Xiao-hu. Primarily study and significance of ET-1 during ischemia with RT-PCR[J]. Journal of Forensic Medicine, 2005, 21(1): 21-23,26 |
| |
| Authors: | Ma Meng-yun Xu Xiao-hu |
| |
| Affiliation: | Shenzhen Institute of Criminal Science and Technology, Shenzhen 518001, China. |
| |
| Abstract: | Objective To explore the relationship between expression of endothelin in heart and ischemia. Methods Using the RT-PCR method, we enquiry changes of ET-1 RNA after 60min ischemia in hearts of rats. Results Although the two group appear positive, they are different significantly, which indicate that ischemia may lead to the increase of ET-1 mRNA. Conclusion The experiment, therefore, pave a way for immunochemical study. |
| |
| Keywords: | ischemia ET-1 RT-PCR rat |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
|
