海兰褐产蛋鸡ALV-J亚型相关纤维肉瘤的鉴别诊断及人工造病试验

在患有典型J亚群白血病肿瘤/血管瘤的34周龄商品代海兰褐产蛋鸡群中,发现1只病鸡在表现皮肤血管瘤的同时还在腹腔中出现了肉瘤样增生物,经病理组织学检测为纤维肉瘤。将该病鸡血浆及肉瘤组织研磨滤过液接种DF1细胞培养,只分离到J亚型白血病病毒(ALV-J),没有分离到A/B亚群ALV、鸡马立克氏病病毒和网状内皮组织增生病病毒。取肉瘤组织研磨滤过液颈部皮下人工接种16只1日龄海兰褐蛋鸡,接种后第8～11天,在接种部位全部出现同样的纤维细胞肉瘤,其中有7只鸡在腹腔内的不同部位出现了相应的肉瘤,且亦能再次分离到ALV-J。取其中8只鸡,对其颈部肿瘤的大小做连续测定,在接种后第11天,肉瘤的直径为11～16mm;第15天迅速长大,直径为23～37mm。人工接种试验结果表明,该产蛋鸡体内的纤维肉瘤是一种与ALV-J感染相关的急性纤维肉瘤,但对诱发急性纤维肉瘤相关的ALV-J中的肿瘤基因还有待深入研究。

Differential diagnosis and bird experiments of fibrosarcoma induced by subgroup J avian leukosis virus in Hy-Line brown layers

A mass of sarcoma was detected in the abdomen of a sick bird with skin hemangioma in a 34-week-old Hy-Line brown commercial layer flock with severe subgroup J avian leukosis.It was diagnosed as fibrosarcoma by histopathological examination.There was only subgroup J avian leukosis virus(ALV) isolated and identified in cell cultures.Subgroups A and B of ALV,Marek’s disease virus and reticuloendotheliosis virus were also tested,but all were negative.Sixteen 1-day-old Hy-Line brown chicken were inoculated subcutaneously in neck with filtered suspension of the sarcoma mass,the similar fibrosarcoma mass was demonstrated and developed from day 8 to 11 after inoculation,and the similar tumors were also found in 7 of 16 birds.ALV-J was isolated and identified again from inoculated birds.Eight of 16 inoculated birds were randomly picked up and counted,the growth of the tumor masses were continuously observed and measured.On day 11 after inoculation,diameter of tumor masses were ranged from 11 to 16 mm,but grew rapidly and reached from 23 to 37 mm in diameter on day 15 after inoculation.The results indicated the sarcoma in the layer was a ALV-J induced acute fibrosarcoma.The oncogene integrated in the mutated ALV-J was to be further identified.