Chelex-100法提取滤纸血痕DNA影响因素的比较

目的改进滤纸血痕DNA提取方法,建立更简便、廉价,适合当前DNA建库需要的提取方法。方法将752份滤纸血痕分成四组,分别按照四种不同的Chelex-100法进行DNA提取并进行比较研究;63份新鲜血痕分别按照两种方法提取并进行对比研究。结果对于陈旧滤纸血痕,四种提取方法的检测成功率无显著差异(P>0.05);对于新鲜血痕,两种提取方法的检测成功率有显著差异(P<0.05)。结论对于建库陈旧滤纸血痕样本的DNA提取可采用不加纯水处理,直接加入Chelex-100的方法进行。

Comparative research of the influence factors of DNA extraction of bloodstain on the filter paper with Chelex-100 method

Objective To improve DNA extraction from bloodstain on the filter paper and to establish a rapid,simple,and cost-effective method for DNA extraction suitable for database construction. Methods Seven hundred and fifty two aged bloodstains on filter paper were randomly divided into four groups. The four different DNA extraction methods were compared with each other,and two DNA extraction methods used for 63 fresh bloodstains on filter paper were also compared with each other. Results There were no statistically significant differences observed among the four DNA extraction methods (P>0.05) for aged bloodstains on filter paper. But the difference between the two DNA extraction methods for fresh bloodstains on filter paper was obviously (P<0.05). Conclusion Extraction of DNA samples from aged bloodstains on filter paper can be accomplished by using Chlex-100 methodology directly with no need to wash the bloodstains.