生物组织中甲胺磷及其异构体的串联质谱法分析

目的建立可用于生物体内甲胺磷及其异构体O,O二甲基氨基硫代磷酸酯检验的高灵敏度方法。方法应用串联质谱法(GCMS/MS),对生物样品中的甲胺磷及其异构体进行检验研究。结果选择质荷比为141的离子为母离子,击发电压为0.8V时,甲胺磷及其异构体二次电离碎片适中,质谱谱图清晰。50%甲胺磷乳油进样量为0.1ng时,甲胺磷及O,O二甲基氨基硫代磷酸酯峰的信噪比分别为436、773。经实验,甲胺磷检测限为20pg。结论所拟方法能有效提高甲胺磷的检测灵敏度。

Detection of methamidophos and its isomer in biological issue by tandem mass spectrometry

Objective To develop a sensitive analytical method for the determination of methamidophos and its isomer (O,O-dimethylamidothiophosphate) in biological issue. Methods Tandem mass spectrometry (GC-MS/MS) was applied to analyze methamidophos and its isomer in biological issue. Results Mother ion at m/z 141 was selected and the voltage of 0.8 V was applied. The quite satisfaction daughter ions and their spectra were obtained. When the amount of 50% methamidophos emulsifiable solution injected is about 0.1ng, the ratios of singal to noise (S/N) for methamidophos and O,O-dimethylamidothiophosphate are 436 and 773 respectively.The detection limit of methamidophos is 20pg. Conclusion The proposed method could raise the detection sensitivity of methamidophos.